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Macromolecular X-ray and Computing X-ray Facility

The facility is located at L141 of the main campus, 3333 Green Bay Road, North Chicago, IL 60064. It supports structural biology projects based on X-ray crystallography, single particle cryo electron microscopy and molecular modeling. Rosalind Franklin University is a member of SER-CAT () with regular access to synchrotron beamlines 22ID and 22BM at Advanced Photon Source.  CryoEM data is collected at National Facilities including S2C2 and NCCAT ().  Linux computing includes a 15TB-4GPU workstation for the cryoEM data processing and several graphics workstations for the x-ray crystallography.  Multiple software suites are kept current by Structural Biology Grid (). Modeling packages include Molecular Operating Environment () and SCHRODINGER (). The facility have equipment as listed below.

Maintenance Information:

  • Confocal mirror 
  • Oxford Cryostream 
  • Rigaku/MSC X-Stream 
  • Rigaku/MSC R-axis IV detector 
  • Rigaku/MSC RU-h2R 
  • Rigaku/MSC RU-h2R 

Useful X-ray DataProcessing guides:

Â鶹ӰÒôMS X-ray Facility Resources:

  • Rigaku RU-h2R 5.4 kW X-ray generator with copper rotating anode 
  • Osmic confocal “green” fine focusing mirrors 
  • R-axis IV++ imaging plate area detector 
  • CCD video system for crystal mounting 
  • MSC X-Stream 2000 cryocooling system 
  • Bruker AXS SMART 6000 CCD detector with 3-circle goniometer 
  • Oxford cryocooling system 
  • TPP Labtech Mosquito crystallization robot 
  • Korima UV crystal detection and visualization system 
  • Liquid nitrogen 
  • Storage liquid nitrogen dewars 
  • Dry-shipping dewars 
  • Cryotools and crystal handling tools 
  • Six large incubators with temperature control 
  • Temperature-controlled room for crystal growth and manipulations 

Â鶹ӰÒôMS CryoEM  Facility Resources:

  • Linux 15TB-4GPU workstation.
  • PELCO easiGlowTM Glow Discharge system.
  • EOL JEM-1230, 120kV Transmission Electron Microscope (TEM) equipped with a high-resolution Hamamatsu ORCA-HR CCD Camera (2624 x 2400 pixel) with AMT Imaging Software for negative stained images.  

Building:  BSB
Room:  L.141
Phone:  847-578-8474